Validasi Kurkumin Hasil Isolasi Rimpang Kunyit Dengan Kromatografi Cair Kinerja Tinggi Photodiode Array Detector

Curcumin is the main compound in turmeric and has problems its stability. The analytical method used to determine curcumin levels by using high performance liquid Chromatography (HPLC) Photodiode Array Detector (PDA). HPLC a separation capability good reproducibility with low detection limit. purpose of this research that isolation curcumine determined HPLC-PDA been validated can be applied as standard quantitative analysis laboratory. validation parameters specified include accuracy, precision, linearity, limits quantification limits. results acetonitrile mobile phase condition : buffer phosphate pH 3,5 (70:30 v/v), flow rate 1 mL/min, wavelength 426 nm showed from had resolution (Rs= 5.7172), linearity (r=0.9991), accuracy (0.5 ppm), presicion at concentration 5 ppm %RSD < 20.0%, BD values also BK are 0.1122 0.3401 ppm. Validation requirements reference